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4 edition of In vitro activities associated with CYP1A1 and CYP1A2 in normal human liver specimens found in the catalog.

In vitro activities associated with CYP1A1 and CYP1A2 in normal human liver specimens

Aleksandra Pastrakuljic

In vitro activities associated with CYP1A1 and CYP1A2 in normal human liver specimens

by Aleksandra Pastrakuljic

  • 178 Want to read
  • 32 Currently reading

Published by National Library of Canada in Ottawa .
Written in English


Edition Notes

Thesis (M.Sc.) -- University of Toronto, 1996.

SeriesCanadian theses = -- Thèses canadiennes
The Physical Object
FormatMicroform
Pagination2 microfiches : negative. --
ID Numbers
Open LibraryOL20822437M
ISBN 100612126285
OCLC/WorldCa46531877

Induction of cyp1a1 is a nonspecific biomarker of aryl hydrocarbon receptor activation: results of large scale screening of pharmaceuticals and toxicants in vivo and in vitro. Mol Pharmacol ; Schweikl H, Taylor JA, Kitareewan S, Linko P, Nagorney D, Goldstein JA. Expression of CYP1A1 and CYP1A2 genes in human liver. Abstract: The aim of this study was to evaluate the in vitro inductive potential of six commonly used trade herbal products on CYP1A2, CYP2D6 and CYP3A4 metabolic activities. Herbal components were .

Cytochrome P 2C8 and CYP3A4/5 are involved in chloroquine metabolism in human liver microsomes Article in Archives of Pharmacal Research 26(8) September with Reads.   Cytochrome PA1 (CYP1A1) induction, a marker of aryl hydrocarbon (Ah) receptor activation, has been associated with carcinogenicity of the environmental agent 2,3,7,8-tetrachlorodibenzo- p -dioxin (TCDD). Consistently, we show that TCDD treatment led to induction of CYP1A1 in responsive human .

The in vitro studies described in this report were designed to evaluate the perpetrator potential of milnacipran. The enzyme-inducing potential of milnacipran was evaluated in three preparations of freshly cultured human hepatocytes, and focused on the major inducible human CYP enzymes, namely CYP1A2.   Schwarz D, Roots I () In vitro assessment of inhibition by natural polyphenols of metabolic activation of procarcinogens by human CYP1A1. Biochem Biophys Res Commun .


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In vitro activities associated with CYP1A1 and CYP1A2 in normal human liver specimens by Aleksandra Pastrakuljic Download PDF EPUB FB2

Although CYP1A2 is a significant isoform involved in PhIP activation in the liver, CYP1A1 plays an equally important role in this process in extrahepatic tissues, such as the by:   In a previous h study, precision-cut rat liver slices were validated as a useful in vitro model for assessing the dose-related induction of CYP1A1 and CYP1A2 in rat liver following exposure to 2, 3,7,8-tetrachlorodibenzo-p-dioxin (TCDD).Cited by: As shown in Table 2, the content of CYP1A1 and CYP1A2 estimated at the fluvoxamine concentration of [xM was very similar in all four livers and in the cell line to the content estimated with isosafrole at 5 u,M.

There- fore, these two simple probes can be used interchangeably for determination of CYP1A1 and CYP1A2 in human by: The presence of CYP1A1 and CYP1A2 was detected both in the liver and kidneys (Fig.

4, Fig. 5, respectively) from all rabbits under study. In the liver, CYP1A1 and CYP1A2 immunoexpression was confined to zone 1 (periportal) and zone 3 (perivenous) regions of the liver Cited by: 2. However, catalytically functional CYP1A2 was detected along with CYP1A1 in histologically normal human lung specimens in other studies (Wei et al., ).

It will be of interest to determine and. CYP1A1 is a major enzyme responsible for the metabolism of granisetron in human liver microsomes. Nakamura H(1), Ariyoshi N, Okada K, Nakasa H, Nakazawa K, Kitada M.

Author information:. The effect of isothiocyanates on CYP1A1 and CYP1A2 activities induced by polycyclic aromatic hydrocarbons in Mcf7 cells May Toxicology in Vitro 23(5)   Microsomes isolated from human liver samples diagnosed as normal, n = 20; steatosis, n = 11; nonalcoholic steatohepatitis (NASH) (fatty liver), n = 10; and NASH (no longer fatty), n = 11 were analyzed for P mRNA, protein, and enzyme activity.

Microsomal CYP1A2, CYP2D6, and CYP2E1 mRNA levels were decreased with NAFLD progression, whereas CYP2A6, CYP2B6, and CYP2C9. Niclosamide also produced clastogenic effects in human lymphocytes in vitro and in vivo and frame-shift mutations in Salmonella sp. Importantly, the mutagenic effect of niclosamide in vitro depends on the presence of the liver.

Human CYP1A1 Structure and Use in Understanding Metabolism 1 Human Cytochrome P 1A1 Structure and Utility In Understanding Drug and Xenobiotic Metabolism* Agnes A. Walsh1, Grazyna.

In the present study, we studied catalytic activities of CYP1A1, CYP1A2 and CYP1B1 in the intact human lung, ex vivo. We also studied the induction responsiveness of the enzymes to B[a]P, diesel. CYP1A2∗1C and CYP1A2∗1D (−delT) appear to be associated with increased clozapine exposure and adverse effects.

Caffeine. The major role that CYP1A2 plays in caffeine metabolism is well established. Caffeine metabolism is one of the best markers of CYP1A2 activity available in vitro and in vivo and has been used as an important tool to study the influence of genetic and nongenetic factors influencing CYP1A2.

The effect of fucoxanthin on the enzyme activity of recombinant human CYPs (CYP1A1, CYP1A2 and CYP3A4) was also analyzed in vitro. Fucoxanthin inhibited the activity of these CYPs, though the effect was weaker compared with known inhibitors, including omeprazole for CYP1A1, α-naphthoflavone for CYP1A2 and ketoconazole for CYP3A4 (Fig.

Few studies have examined the potential of carotenoids in the inhibition of CYP enzyme activity. CYP1A1 and CYP1A2 are involved in both detoxification and metabolic activation of xenobiotics. Human CYP1A1 (hCYP1A1) and hCYP1A2 exist in a head-to-head orientation in chromosome 15 with the.

Induction of CYP1A1 and CYP1A2 expressions by prototypic and atypical inducers in human lung Article in Cancer Letters (1) May with 36 Reads How we measure 'reads'.

Comparison of CYP Activities from Human Liver Microsome Pools Based on Weight, Gender and Age Abstract / Introduction LaHoma Easterwood1, Matt Palmer1, Kevin Lehnert1, Jason Wright1, Lyndee.

Cell culture cytochrome P drug development enzyme induction human hepatocytes in vitro liver microsomes This is a preview of subscription content, log in to check access.

Springer Nature is. to TCDD is the enhanced expression of CYP1A1 and CYP1A2. In contrast, knowledge of the response of CYP1B1 is limited. CYP1B1 RNA is detectable in a variety of human tissues (5,13) and inducible by TCDD treatment in vitro.

Human hepatocyte systems for in vitro toxicology analysis a wide range of different strategies for in vitro human liver models have been basal drug-metabolizing enzyme activities (CYP1A2. Summary of CYP1A1 (CP11, CYP1, P, PC, PDX) expression in human tissue. General cytoplasmic expression.

EROD, MROD, and 6β-TH activities in rats on high-fat diet exceeded those in rats on fat-free diet by 64, 58, and %, respectively. Addition of indolecarbinol to the diet led to an increase in CYP1A1, CYP1A2, and CYP3A1 activities .Abstract. Major hepatic cytochrome P activities (CYP1A2, CYP2A6, CYP2B6, CYP2C9, CYP2C19, CYP2D6, CYP2E1, and CYP3A4) can be simultaneously examined in human hepatocytes by .Correlation of CYP‐ and NADPH:CYP reductase‐linked enzyme activities in human hepatic microsomes with DNA adduct formation by AAI.

Catalytic activities known to be associated with specific CYP enzymes (CYP1A1/2, 2A6, 2C9, 2D6, 2E1 and 3A4) were analyzed in all 8 human hepatic microsomal preparations (Table I).

CYP2B6 activities .